KMID : 0364219590020020010
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Korean Journal of Zoology 1959 Volume.2 No. 2 p.10 ~ p.14
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Studies on the Composition of Amino Acid in Retina and Lens Body of the Frogs
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Kang Sung-Ho
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Abstract
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The retinae and the lens bodies of the frogs were hydrolyzed with 20% hydrochloric acid, and their amino acids were separated by paper chromatography. As a result of it the following were confirmed : (1) The retinae and the lens bodies were the same incomposition, and aspartic acid, glutamic acid, serine ,tyrosine, glycine, lysine arginine, threonine, alanine, histidine, proline, methionine, valine , phenylalanine, leucine, and two unknown substances were separated. (2) The free amino acids in the retinae were extracted with 80% ethyl alcohol and then separated by paper chromatography. Though their separation was not so definite , serine, gultaminc acid, and glycine were always separated regardless of the amount of the sample. When the amount of the smaple was enough , ¥â-alanine , ¥ã-amino butyric acid and methionine +valine were also separated. (2) The free amino acids in the retinae were extracted with 80% ethyl alcohol and then separated by paper chromatography. Though their separation was not so definite, serine, glutamic acid, and glycine were always separated regardless of the amount of the sample . When the amount of the sample was enough, ¥â-alanine , ¥ã-amino butyric acid and methionine + valine were also separated.
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